Phenolic content and antioxidant action in needle extracts from Pinus cooperi, P. durangensis, P. engelmanii and P. teocote

Authors

  • María de los Ángeles Sáenz-Esqueda Instituto Tecnológico de Durango. Departamento de Ingeniería Química y Bioquímica
  • Martha Rosales-Castro Instituto Politécnico Nacional. CIIDIR Unidad Durango
  • Nuria Elizabeth Rocha-Guzmán Instituto Tecnológico de Durango. Departamento de Ingeniería Química y Bioquímica
  • José Alberto Gallegos-Infante Instituto Tecnológico de Durango. Departamento de Ingeniería Química y Bioquímica
  • Rubén Francisco González-Laredo Instituto Tecnológico de Durango. Departamento de Ingeniería Química y Bioquímica

DOI:

https://doi.org/10.21829/myb.2010.1631165

Keywords:

ABTS, Antioxidants, DPPH, LDL, needles, polyphenols

Abstract

Coniferous trees are a vast resource in Durango, Mexico forests and a potential nontimber source of these chemicals, thus, our study for analyzing phenolic content and biological activity in needle extracts from Pinus cooperi (COO), P. durangensis (DUR), P. engelmanii (ENG) and P. teocote ( TEO). Pine needles were dewaxed with hexane and extracted with aqueous acetone and methanol. DUR and ENG extracts showed the higher yields, while acetone TEO and methanol ENG extracts showed the highest catechin equivalent (CE) contents. A partial chromatographic purification on acetone TEO extracts produced five fractions, the third one (F3-TEO) had the highest phenolic content (489,6 CE). In condensed tannins contents, acetone and methanol TEO extracts, as well fraction F3-TEO were the best (24,3, 15.7 and 44,4 mg/g extract, respectively). In DPPH activity, the more effective CE50 were obtained from TEO acetone extract (223,5 ppm) and fractions F3-TEO (145,0 ppm) and F4-TEO (150,8 ppm). In ABTS assay, TEO acetone (27,7%) and methanolic (18,2%) extracts, and fractions F3-TEO (60,1%) and F4-TEO (55,8%) were the best. In LDL assay the highest inhibitory activity was shown by acetone extracts, particularly ENG (99,4%) at 90 ppm. Therefore acetone extracts from TEO and ENG may have utilization as a potential source of bioactive phenolics.

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Author Biographies

María de los Ángeles Sáenz-Esqueda,

Instituto Tecnológico de Durango. Departamento de Ingeniería Química y Bioquímica

Instituto Tecnológico de Durango. Departamento de Ingeniería Química y Bioquímica. Felipe Pescador 1830 Ote. 34080 Durango, Dgo. México

Martha Rosales-Castro,

Instituto Politécnico Nacional. CIIDIR Unidad Durango

CIIDIR Unidad Durango. Instituto Politécnico Nacional. Becaria de COFAA-IPN

Nuria Elizabeth Rocha-Guzmán,

Instituto Tecnológico de Durango. Departamento de Ingeniería Química y Bioquímica

Instituto Tecnológico de Durango. Departamento de Ingeniería Química y Bioquímica. Felipe Pescador 1830 Ote. 34080 Durango, Dgo. México

José Alberto Gallegos-Infante,

Instituto Tecnológico de Durango. Departamento de Ingeniería Química y Bioquímica

Instituto Tecnológico de Durango. Departamento de Ingeniería Química y Bioquímica. Felipe Pescador 1830 Ote. 34080 Durango, Dgo. México

Rubén Francisco González-Laredo,

Instituto Tecnológico de Durango. Departamento de Ingeniería Química y Bioquímica

Instituto Tecnológico de Durango. Departamento de Ingeniería Química y Bioquímica. Felipe Pescador 1830 Ote. 34080 Durango, Dgo. México

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Published

2016-08-30

How to Cite

Sáenz-Esqueda, M. de los Ángeles, Rosales-Castro, M., Rocha-Guzmán, N. E., Gallegos-Infante, J. A., & González-Laredo, R. F. (2016). Phenolic content and antioxidant action in needle extracts from Pinus cooperi, P. durangensis, P. engelmanii and P. teocote. Madera Y Bosques, 16(3), 37–48. https://doi.org/10.21829/myb.2010.1631165
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Vol. 16 No. 3 (2010): Otoño 2010

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Research papers

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Madera y Bosques by Instituto de Ecología, A.C. is licensed under a Creative Commons Atribution-Non-Commercial-Share-alike 4.0 International.

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